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About This Item
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
agarose conjugate
Clone:
M2, monoclonal
Application:
IP, affinity chromatography
Citations:
1232
conjugate
agarose conjugate
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous glycerol solution
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
matrix
(4% agarose bead; 45-165μm bead size)
isotype
IgG1
capacity
>0.6 mg/mL, resin binding capacity (FLAG-BAP)
shipped in
wet ice
storage temp.
−20°C
General description
Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.
Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide
Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide
FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide
Immunogen
DYKDDDDK
Application
Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Physical form
Suspension in buffered saline containing azide as preservative and 50% glycerol
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
FLAG® tag, 3x FLAG®, DYKDDDDK tag
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001