G4142
β-Galactosidase from bovine testes
ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)
Synonym(s):
β-D-Galactoside galactohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
1.0-3.0 units/mg protein (modified Warburg-Christian)
Biological source:
bovine
biological source
bovine
Quality Level
form
ammonium sulfate suspension
specific activity
1.0-3.0 units/mg protein (modified Warburg-Christian)
foreign activity
α- and β-glucosidase ≤0.2%, α-fucosidase ≤0.2%, α-galactosidase ≤0.2%, α-mannosidase ≤0.2%, β-N-acetylglucosaminidase ≤1.0%
storage temp.
2-8°C
Application
β-Galactosidase from bovine testes was used for coupling to Sepharose.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Physical form
Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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