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L2500

L-Lactic Dehydrogenase from rabbit muscle

Name: <SC>L</SC>-Lactic Dehydrogenase from rabbit muscle
Brand: SIGMA

Type II, ammonium sulfate suspension, 800-1,200 units/mg protein

Synonym(s):

Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD

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About This Item

CAS Number:

9001-60-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-617-8

MDL number:

MFCD00131466

EC Number:

1.1.1.27 (BRENDA, IUBMB)

Specific activity:

800-1,200 units/mg protein

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Properties

type

Type II

Quality Level

200

form

ammonium sulfate suspension

specific activity

800-1,200 units/mg protein

mol wt

140 kDa

foreign activity

pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .

Application

L-Lactic Dehydrogenase from rabbit muscle has been used:

for protein-binding measurement
to detect the action of oxaloacetate decarboxylase
to determine serum L- and D-lactate

Biochem/physiol Actions

Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ solution, pH 6.0

Analysis Note

Protein determined by biuret.

Other Notes

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

ppe

Eyeshields, Gloves, type N95 (US)

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