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N2765

4-Nitrophenyl phosphate disodium salt hexahydrate

Name: 4-Nitrophenyl phosphate disodium salt hexahydrate
Brand: SIGMA

chromogenic, tablet

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

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About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂· 6H₂O

CAS Number:

333338-18-4

Molecular Weight:

371.14

NACRES:

NA.83

PubChem Substance ID:

24897592

UNSPSC Code:

12352204

EC Number:

224-246-5

MDL number:

MFCD00066288

Beilstein/REAXYS Number:

5324661

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Properties

Product Name

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

form

tablet

Quality Level

300

solubility

water, high purity: 0.25 tablet/mL, clear, colorless

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:

as a substrate for alkaline phosphatase coupled anti-rabbit antibody in enzyme-linked immunosorbent assay (ELISA)
as a substrate for the mouse secondary antibody linked to alkaline phosphatase in immunoenzymometric assay
in ELISA to analyze the presence of antibodies to Pseudomonas aeruginosa in animal serum sample

Preparation Note

Contains 20 mg substrate per tablet

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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