Anti-SC-35 Antibody, clone 1SC-4F11

SC-35 is also known as Splicing factor SC35 and Splicing factor, arginine/serine-rich 2. This protein is encoded by the SFRS2 gene and is a member of the splicing factor SR family. SC-35 is involved in pre-mRNA splicing and is found in bodies in the nucleus that are highly enriched in poly(A) RNA. These nuclear bodies are referred to as speckles, SC 35 domains, or splicing factor compartments (SFCs). The purpose of these bodies is still widely disputed. Speckles correspond largely to, if not entirely to, ultra structures called inter-chromatin granule clusters (IGCs). SC3-35 is required for pre-mRNA splicing events and is highly involved in spliceosomal interaction and assembly. SC-35 is necessary for assembly of the ATP-dependent splicing complex. It is also necessary for the ATP-dependent communication between pre-mRNA and U1 and U2 snRNPs.

~ 35 kDa

Epitope: Unknown

Ovalbumin-conjugated linear peptide corresponding to human SC35.

Anti-SC-35 Antibody, clone 1SC-4F11 is a Mouse Monoclonal Antibody for detection of SC-35 also known as Protein PR264, Splicing factor SC35 & has been validated in WB, ICC.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SC35 in NIH/3T3 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

This antibody recognizes SC35.

Unpurified

Unpurified mouse monoclonal IgG1κ preservative-free ascites.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
A431 cell lysate

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Sc35 on 10 µg of A431 cell lysate.

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