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Merck

CP70

Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)

liquid, clone FBN11, Calbiochem®

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NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
FBN11, monoclonal
Species reactivity:
rat, human, mouse
Application:
Citations:
4

Documentos clave

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Nombre del producto

Anti-Fibronectin (Ab-3) Mouse mAb (FBN11), liquid, clone FBN11, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

FBN11, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Frozen Sections (0.5-1 µg/mL)
Immunoblotting (0.5-1 µg/mL)
Immunofluorescence
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/mL, heat pre-treatment required))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)

Analysis Note

Positive Control
SW 156 cells or normal kidney tissue

Application

Frozen Sections (0.5-1 µg/ml)

Immunoblotting (0.5-1 µg/ml)

Immunofluorescence (see comments)

Immunoprecipitation (2 µg/mg protein lysate)

Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.

Immunogen

recombinant human fibronectin

Other Notes

Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.
Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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R Schaal-Jensen et al.
Journal of thrombosis and haemostasis : JTH, 5(10), 2070-2078 (2007-07-26)
In cynomolgus monkeys, suprapharmacological doses of clotting recombinant factor XIII (rFXIII) cause a generalized coagulopathy, associated with formation of circulating high molecular weight protein complexes (HMEX). HMEX consist of plasma protein substrates cross-linked by FXIII transglutaminase activity. To characterize HMEX
Ismaïl Hendaoui et al.
Matrix biology : journal of the International Society for Matrix Biology, 40, 46-53 (2014-09-10)
Whisker follicles have multiple stem cell niches, including epidermal stem cells in the bulge as well as neural crest-derived stem cells and mast cell progenitors in the trabecular region. The neural crest-derived stem cells are a pool of melanocyte precursors.
Yu-Jen Chiu et al.
International journal of oncology, 52(5), 1465-1478 (2018-03-24)
Osteosarcoma is the most common primary malignancy of the bone and is characterized by local invasion and distant metastasis. Over the past 20 years, long-term outcomes have reached a plateau even with aggressive therapy. Overexpression of insulin-like growth factor 1 receptor
Nicole Vincent Jordan et al.
Molecular and cellular biology, 33(15), 3011-3025 (2013-05-30)
We previously identified a gene signature predicted to regulate the epithelial-mesenchymal transition (EMT) in both epithelial tissue stem cells and breast cancer cells. A phenotypic RNA interference (RNAi) screen identified the genes within this 140-gene signature that promoted the conversion

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