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Merck

CP74

Anti-Vinculin Mouse mAb (V284 or VLN01)

liquid, Calbiochem®, from mouse

Sinónimos:

Anti-Vinculin antibody

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NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
V284, monoclonal
VLN01, monoclonal
Species reactivity:
human
Application:
Citations:
21

Documentos clave

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Nombre del producto

Anti-Vinculin Mouse mAb (V284 or VLN01), liquid, Calbiochem®, from mouse

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

V284, monoclonal
VLN01, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Immunoblotting (1 µg/mL, chemiluminescence)
Immunoprecipitation (2 µg/mL lysate)
Paraffin Sections (4-8 µg/mL, heat pre-treatment required))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... VCL(7414)

Analysis Note

Negative Control
Normal mouse serum
Positive Control
Skin cells or tissue

Application


Immunoblotting (1 g/ml, chemiluminescence)
Immunoprecipitation (2 g/ml lysate)
Paraffin Sections (4-8 g/ml, heat pre-treatment required)

Disclaimer

Toxicity: Standard Handling (A)

General description

Protein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma SP2/0 cells. Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins.
Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.
This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.

Immunogen

Human
semi-purified, human vinculin

Other Notes

Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol.79, 961.
Bakolitsa, C., et al. 1999 Cell99, 603.
Rudiger, M., et al. 1998 Bioessays20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem.274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil.17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton16, 1.
This antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Alexey V Bazarov et al.
Cell cycle (Georgetown, Tex.), 11(5), 1008-1013 (2012-02-16)
RB family proteins pRb, p107 and p130 have similar structures and overlapping functions, enabling cell cycle arrest and cellular senescence. pRb, but not p107 or p130, is frequently mutated in human malignancies. In human fibroblasts acutely exposed to oncogenic ras
Tim Lahm et al.
American journal of physiology. Lung cellular and molecular physiology, 311(2), L375-L388 (2016-06-12)
17β-Estradiol (E2) exerts protective effects on right ventricular (RV) function in pulmonary arterial hypertension (PAH). Since acute exercise-induced increases in afterload may lead to RV dysfunction in PAH, we sought to determine whether E2 allows for superior RV adaptation after
Rebekah L Mokry et al.
mBio, 11(6) (2020-12-17)
Nitric oxide is a versatile and critical effector molecule that can modulate many cellular functions. Although recognized as a regulator of infections, the inhibitory mechanism of nitric oxide against human cytomegalovirus (HCMV) replication remains elusive. We demonstrate that nitric oxide
Gesche Frohwitter et al.
Oncology letters, 12(1), 107-113 (2016-06-28)
Squamous cell carcinoma (SCC) of the oral cavity is a morphological heterogeneous disease. Various cytokeratin (CK) expression patterns with different prognostic values have been described, but little is known concerning the underlying biological cell mechanisms. Therefore, the present study investigated
Vaibhav Sidarala et al.
Nature communications, 13(1), 2340-2340 (2022-04-30)
The dynamin-like GTPases Mitofusin 1 and 2 (Mfn1 and Mfn2) are essential for mitochondrial function, which has been principally attributed to their regulation of fission/fusion dynamics. Here, we report that Mfn1 and 2 are critical for glucose-stimulated insulin secretion (GSIS)
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