Anti-Integrin beta 1 (CD29) Antibody

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Human keratinocytes derived from skin.

Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.

Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).

ELISA: A representative lot of this antibody was used in ELISA.

Immunocytochemistry: A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).

Functional Activity Assay: A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).

Anti-Integrin β1 Antibody, clone P5D2 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IC, IH & IP.

Research Category
Cell Structure

Research Sub Category
Integrins

Reacts with Human Integrin Beta 1.

Format: Purified

Protein A purified

Purified immunoglobulin in 0.02M PB, 0.25M NaCl, pH 7.6, 0.1% sodium azide.

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry Analysis:
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.

Control
Human tonsil, human skin tissue

A431 & HeLa Cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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