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Merck

MAB2160

Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3

ascites fluid, clone 1C3, Chemicon®

Sinónimos:

FMRP

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1C3, monoclonal
Application:
ELISA, ICC, IHC (p), WB
Species reactivity:
mouse, human, rat
Citations:
73
Technique(s):
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
Uniprot accession no.:
Q06787

Documentos clave

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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1C3, monoclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

isotype

IgG1κ

suitability

not suitable for flow cytometry, not suitable for immunoprecipitation

NCBI accession no.

NM_002024.3

UniProt accession no.

Q06787

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... FRAXA(2477), FRAXA(108684022)

Analysis Note

Control
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate
Evaluated by Western Blot on Mouse E17 spinal cord lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.

Application

Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3 detects level of Fragile X Mental Retardation Protein & has been published & validated for use in ELISA, IC, IH, IH(P) & WB.
Immunohistochemistry:
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.

Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

RNA Binding Protein (RBP)

Biochem/physiol Actions

Human and mouse FMRP. The epitope is localized in the N-terminal half of FMRP. Cross reaction with FXR protein may be detected in cases of high expression of the latter proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
~71 kDa

Immunogen

Fusion protein with a full length FMRP (human).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Ascites mouse monoclonal IgG1k fluid containing no preservatives
Unpurified

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function.
J C Darnell, K B Jensen, P Jin, V Brown, S T Warren, R B Darnell
Cell null
The RNA-binding protein fragile X-related 1 regulates somite formation in Xenopus laevis.
Huot, ME; Bisson, N; Davidovic, L; Mazroui, R; Labelle, Y; Moss, T; Khandjian, EW
Molecular Biology of the Cell null
C H Lin et al.
Journal of biochemistry, 128(3), 493-498 (2000-08-31)
Arginine methylation in RNA-binding proteins containing arginine- and glycine-rich RGG motifs is catalyzed by specific protein arginine N-methyltransferase in cells. We previously showed that lymphoblastoid cells grown in the presence of an indirect methyltransferase inhibitor, adenosine dialdehyde (AdOx), accumulated high
Sally M Till et al.
Human molecular genetics, 24(21), 5977-5984 (2015-08-06)
Recent advances in techniques for manipulating genomes have allowed the generation of transgenic animals other than mice. These new models enable cross-mammalian comparison of neurological disease from core cellular pathophysiology to circuit and behavioural endophenotypes. Moreover they will enable us
Rapid antibody test for fragile X syndrome.
Willemsen, R, et al.
Lancet, 345, 1147-1148 (1995)
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Contenido relacionado

Anti-Fragile X Mental Retardation Protein, clone 1C3 - Data Sheet
RNA-Binding Protein Immunoprecipitation

All eukaryotic organisms require tight regulation of gene expression for complex processes such as development, differentiation, cell specification, and responses to environmental stimuli. Many genes are regulated post-transcriptionally, in addition to transcriptional mechanisms of gene regulation. RNA-binding proteins (RBPs) are essential for post-transcriptional gene regulation, linking transcription and translation in many processes including transcription, splicing, export, rate of translation and turnover. In all of these events, RBPs coordinate regulation of the amount of protein produced from mRNA transcripts.

Número de artículo de comercio global

SKUGTIN
MAB216004053252266911

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