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Merck

114391

Bromophenol Blue

ACS reagent

Sinónimos:

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form

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Fórmula empírica (notación de Hill):
C19H10Br4O5S
Número CAS:
115-39-9
Peso molecular:
669.96
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24847169
EC Number:
204-086-2
Beilstein/REAXYS Number:
61698
MDL number:
MFCD00005875

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InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

grade

ACS reagent

form

powder

technique(s)

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

mp

273 °C (lit.)

solubility

ethanol: 0.1%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCG4635
BCCG0495
BCCD6533
SHBL2679
SHBL1423

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