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Merck

68724

Fast Green FCF

analytical standard

Sinónimos:

Food green 3

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Fórmula lineal:
C37H34N2O10S3Na2
Número CAS:
2353-45-9
Peso molecular:
808.85
UNSPSC Code:
85151701
NACRES:
NA.24
E Number:
E143
PubChem Substance ID:
329761116
EC Number:
219-091-5
MDL number:
MFCD00013053
Colour Index Number:
42053
Beilstein/REAXYS Number:
5718212

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Nombre del producto

Fast Green FCF, analytical standard

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

grade

analytical standard

assay

≥98.0% (HPLC)

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

290 °C (dec.) (lit.)

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

Quality Level

100

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Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

General description

Fast Green FCF is a synthetic organic dye, which belongs to the triphenylmethane group. It can be found in a wide range of applications in food and beverage industry. It can also be used in scientific research for the staining of proteins and identification of extracellular recording or injection sites in electrophysiological experiments.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H335,H341

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
BCCG5753
BCCB8461
BCBV4344

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Visite la Librería de documentos

Nonlinear responses and optical limiting behavior of fast green FCF dye under a low power CW He?Ne laser irradiation
Jamshidi-Ghaleh K, et al.
Optics Communications, 271, 551-554 (2007)
Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons
Hooft V.AJ
Neuroscience Letters, 318, 163-165 (2002)
Diane G Elliott et al.
Diseases of aquatic organisms, 84(2), 139-150 (2009-05-30)
A rapid staining procedure for detection of recent skin and fin injuries was tested in juvenile Chinook salmon Oncorhynchus tshawytscha. Immersion of anesthetized fish for 1 min in aerated aqueous solutions of the synthetic food dye fast green FCF (Food
E Ngang et al.
Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan, 42(5), 298-303 (2002-01-05)
HPLC analysis revealed that eight subsidiary colors existed in commercial Food Green No. 3 (fast green FCF, FD & C Green No. 3). Among them, four subsidiary colors C, F, G, and H were isolated by using preparative HPLC and
Daniel H O'Connor et al.
Journal of neurophysiology, 94(2), 1565-1573 (2005-04-01)
In populations of synapses, overall synaptic strength can undergo either a net strengthening (long-term potentiation) or weakening (long-term depression). These phenomena have distinct induction pathways, but the functional outcome is usually measured as a single lumped quantity. In hippocampal CA3-CA1
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