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Merck

A0262

Lithium potassium acetyl phosphate

≥85% (elemental analysis), powder

Sinónimos:

Acetyl phosphate lithium potassium salt

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Fórmula lineal:
CH3COOP(O)(OK)(OLi)
Número CAS:
94249-01-1
Peso molecular:
184.06
NACRES:
NA.32
PubChem Substance ID:
24890415
UNSPSC Code:
12352204
EC Number:
304-378-0
MDL number:
MFCD00002134
Beilstein/REAXYS Number:
5689718

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Nombre del producto

Lithium potassium acetyl phosphate, high-energy phosphate donor

InChI

1S/C2H5O5P.K.Li/c1-2(3)7-8(4,5)6;;/h1H3,(H2,4,5,6);;/q;2*+1/p-2

InChI key

RLQMPLKXFIXRCV-UHFFFAOYSA-L

assay

≥85% (elemental analysis)

form

powder

mp

>300 °C (lit.)

solubility

water: 25 mg/mL, clear, colorless

storage temp.

−20°C

Quality Level

200

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Application

Lithium potassium acetyl phosphate:
  • has been used as a substrate for CpxR (transcription factor) in x-ray crystallography to soak CpxR receiver domain (CpxRRD) crystals to obtain the phosphorylated form
  • may be used as a phosphate (P) donor in a study to screen a wide range of natural and synthetic organic phosphate donors with several enzymes on various hydroxyl?compounds for the improved enzymatic synthesis of phosphate monoesters
  • has been used to set up premixA for in vitro translation reaction

Biochem/physiol Actions

Lithium potassium acetyl phosphate is commonly used as the source for acetyl phosphate. So acetyl phosphate can be used as a suitable alternative to replace inorganic oligophosphates in phosphatase-catalyzed transphosphorylation to reduce inorganic monophosphate by-product formation.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

hcodes

H314,H361

Hazard Classifications

Eye Dam. 1 - Repr. 2 - Skin Corr. 1B

Clase de almacenamiento

8B - Non-combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
057K5317V
BCBC3616V
BCBC2020V
BCBC3616
BCBC2020

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Sebastian R Schmidl et al.
Nature chemical biology, 15(7), 690-698 (2019-05-22)
Two-component systems (TCSs) are the largest family of multi-step signal transduction pathways and valuable sensors for synthetic biology. However, most TCSs remain uncharacterized or difficult to harness for applications. Major challenges are that many TCS output promoters are unknown, subject
Juan Andres Imelio et al.
Bio-protocol, 7(16), e2510-e2510 (2017-08-20)
We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in E. coli, ensuring an excess of the regulator
Emiko Kinoshita-Kikuta et al.
PloS one, 10(7), e0132598-e0132598 (2015-07-08)
Hybrid sensor kinase, which contains a histidine kinase (HK) domain, a receiver domain, and a histidine-containing phosphotransmitter (HPt) domain, conveys signals to its cognate response regulator by means of a His-Asp-His-Asp phosphorelay. We examined the multistep phosphorelay of a recombinant
Coilín Boland et al.
Cellular and molecular life sciences : CMLS, 71(24), 4895-4910 (2014-07-12)
Membrane proteins are key elements in cell physiology and drug targeting, but getting a high-resolution structure by crystallographic means is still enormously challenging. Novel strategies are in big demand to facilitate the structure determination process that will ultimately hasten the

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