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Merck

B8434

Anti-BrdU antibody, Mouse monoclonal

clone BU-33, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-BrdU antibody produced in mouse, Anti Brdu, Brdu Antibody, Brdu Antibody - Anti-BrdU antibody, Mouse monoclonal, Anti-Bromodeoxyuridine

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UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00164550
Conjugate:
unconjugated
Clone:
BU-33, monoclonal
Application:
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Species reactivity:
wide range
Citations:
185
Technique(s):
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU

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Nombre del producto

Anti-BrdU antibody, Mouse monoclonal, clone BU-33, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture
purified immunoglobulin

antibody product type

primary antibodies

clone

BU-33, monoclonal

form

buffered aqueous solution

species reactivity

wide range

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

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Application

Anti-BrdU antibody has been used in:
  • BrdU labeling
  • immunohistochemistry
  • immunostaining
  • BrdU assay
Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody:
  • to detect 5-fluorouridine-labelled RNA
  • to detect 5-bromo-2′-deoxyuridine (BrdU) labelled immunoreactive cells
  • against BrdU labelled rat retinal sections

Biochem/physiol Actions

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle.
Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Immunogen

bromodeoxyuridine conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

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Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
099M4772V
039M4889V
22181210
019M4791V
118M4851V

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Seifert R.
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The Journal of Biological Chemistry, jbc-M114 (2014)
Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure
Hong Jie, et al.
Experimental Eye Research, 17-26 (2013)
Hughes D and Mehmet H
Cell Proliferation and Apoptosis (2003)
Fang Wang et al.
Biology of reproduction, 85(4), 702-713 (2011-06-24)
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