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Merck

C4710

Monoclonal Anti-Cyclin A antibody produced in mouse

clone CY-A1, ascites fluid

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UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
Conjugate:
unconjugated
Clone:
CY-A1, monoclonal
Application:
ARR, ICC, WB
Citations:
44
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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CY-A1, monoclonal

mol wt

antigen 60 kDa

contains

15 mM sodium azide

species reactivity

mouse, human, monkey, bovine

technique(s)

immunocytochemistry: suitable, microarray: suitable, western blot: 1:1,000 using a mouse NIH 3T3 cell extract

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... CCNA2(890)
mouse ... Ccna2(12428)

General description

Cyclin A is a nuclear protein during S phase and it binds both cdk2 and cdc2. The availability of monoclonal antibody reacting specifically with cyclin A enables the subcellular detection and localization of cyclin A and the measurement of relative differences in cyclin A levels as a function of cell cycle phase.

Immunogen

recombinant bovine cyclin A.

Application

Monoclonal Anti-Cyclin A antibody produced in mouse is suitable for:
  • immunocytochemistry
  • microarray
  • western blotting at a dilution of 1:1,000 using a mouse NIH 3T3 cell extract

Biochem/physiol Actions

The antibody recognizes an epitope present in amino acids 162-433 of bovine cyclin A. No cross-reactivity is detected with other cyclins (cyclin B, D, or E).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Clase de almacenamiento

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Andres Dekanty et al.
The Journal of biological chemistry, 281(10), 6136-6143 (2005-11-18)
Leukemia inhibitory factor (LIF) and oncostatin M (OSM) induce DNA synthesis in Swiss 3T3 cells through common signaling mechanism(s), whereas other related cytokines such as interleukin-6 and ciliary neurotrophic factor do not cause this response. Induction of DNA replication by

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