F4042
Monoclonal ANTI-FLAG® M5 antibody produced in mouse
clone M5, purified immunoglobulin, buffered aqueous solution
Sinónimos:
Anti-ddddk, Anti-dykddddk
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Permítanos ayudarleNombre del producto
Monoclonal ANTI-FLAG® M5 antibody produced in mouse, clone M5, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M5, monoclonal
form
buffered aqueous solution
purified by
using Protein A
species reactivity
all
concentration
2-5 mg/mL
technique(s)
western blot (chemiluminescent): 10 μg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
Quality Level
Categorías relacionadas
Application
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
General description
Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.
Method of purification - Protein A
Method of purification - Protein A
Immunogen
FLAG; peptide sequence DYKDDDDK
Other Notes
Antibody is not calcium dependent.
Physical form
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)
Preparation Note
Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Clase de almacenamiento
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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