G7396
β-Glucuronidase from Escherichia coli
Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
Sinónimos:
β-D-Glucuronide glucuronosohydrolase
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
1,000,000-5,000,000 units/g protein (30 min assay)
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Permítanos ayudarletype
Type IX-A
form
lyophilized powder
specific activity
1,000,000-5,000,000 units/g protein (30 min assay)
mol wt
290 kDa
composition
Protein, 30-70% biuret
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
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about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Contains buffer salts and stabilizer.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
signalword
Danger
Clase de almacenamiento
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
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R Meatherall
Journal of analytical toxicology, 18(7), 382-384 (1994-11-01)
Conditions for the enzymatic hydrolysis of benzodiazepine conjugates were systematically examined. Optimal recovery of the free drugs occurs when 1 mL of urine buffered to pH 4.5 is incubated with 5000 U of Helix pomatia beta-glucuronidase at 56 degrees C
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine
Health Laboratory Science, 50, 286-289 (2004)
Maria Papageorgiou et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 35(10), 2032-2044 (2018-08-18)
Deoxynivalenol (DON) is a Fusarium toxin, to which humans are frequently exposed via diet. Although the elderly are speculated to be sensitive to the toxic effects of DON as a result of age-related conditions, disease and altered DON metabolism, there
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There is a critical need to better understand the patterns, levels and combinatory effects of exposures we are facing through our diet and environment. Mycotoxin mixtures are of particular concern due to chronic low dose exposures caused by naturally contaminated
Amanda Cano et al.
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Temporal lobe epilepsy is the most common type of pharmacoresistant epilepsy in adults. Epigallocatechin-3-gallate has aroused much interest because of its multiple therapeutic effects, but its instability compromises the potential effectiveness. PEGylated-PLGA nanoparticles of Epigallocatechin-3-gallate were designed to protect the
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