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Merck

K1757

Keratinocyte Growth Factor human

≥95% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, powder, suitable for cell culture

Sinónimos:

hFGF-7, hKGF, FGF-7, KGF

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Número CAS:
UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥95% (SDS-PAGE and HPLC)
Form:
powder
Mol wt:
18.9 kDa
Impurities:
1 EU/μg
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Nombre del producto

Keratinocyte Growth Factor human, KGF, recombinant, expressed in E. coli, powder, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE and HPLC)

form

powder

potency

0.1-15 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

18.9 kDa

packaging

pkg of 10 and 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

1 EU/μg

UniProt accession no.

storage temp.

−20°C

Quality Level

Gene Information

human ... FGF7(2252)

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Analysis Note

Determined by a cell proliferation assay using 4MBr-5 cells.

Application

Keratinocyte Growth Factor human has been used:
  • as a component of epithelial growth medium
  • as a positive control for antigen to determine the activity of the antibody
  • to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions

Biochem/physiol Actions

Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium. It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells. KGF contributes to the survival of cells under stress conditions. KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.

General description

Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids, belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines, mesenchymal cells but absent from normal glial cells and epithelial cell lines.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Characteristics of MIC-1 antlerogenic stem cells and their effect on hair growth in rabbits.
Cegielski M, et al.
In Vivo, 27(1), 97-106 (2013)
P Gillis et al.
Journal of cell science, 112 ( Pt 12), 2049-2057 (1999-05-26)
Keratinocyte growth factor (KGF), also called fibroblast growth factor-7, is widely known as a paracrine growth and differentiation factor that is produced by mesenchymal cells and has been thought to act specifically on epithelial cells. Here it is shown to
Keratinocyte growth factor (KGF) is required for postnatal thymic regeneration
Alpdogan O, et al.
Blood, 107(6), 2453-2460 (2006)
Stimulation of in vivo angiogenesis by in situ crosslinked, dual growth factor-loaded, glycosaminoglycan hydrogels
Elia R, et al.
Biomaterials, 31(17), 4630-4638 (2010)
Synergism between keratinocyte growth factor and carboxymethyl chitosan reduces pericardial adhesions
Lopes JB, et al.
The Annals of Thoracic Surgery, 90(2), 566-572 (2010)

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