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Merck

L3126

Lipase from porcine pancreas

Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

Sinónimos:

Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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Número CAS:
9001-62-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
Número CE:
3.1.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00131509
Specific activity:
≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Biological source:
Porcine pancreas

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InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

biological source

Porcine pancreas

type

Type II

form

lyophilized (cake)

specific activity

≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

composition

protein, ≥20%

storage temp.

2-8°C

Quality Level

200

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Analysis Note

Crude (Steapsin). Contains amylase and protease activity.
Protein determined by biuret.

Application

Lipase from porcine pancreas has been used:
  • for the analysis of fatty acid at the 2-position of triglycerides.
  • to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
  • as a component of dissolution media to study the dissolution profiles of theophylline.
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
SLCG8579
SLCJ2799
SLCD5418
SLCD5409
SLBZ7254

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M T Andonaegui et al.
Drug development and industrial pharmacy, 25(11), 1199-1203 (1999-12-22)
Dissolution profiles of theophylline (TP) from three types of sustained-release (SR) matrix tablets (plastic [PL], lipid [LP], and hydrophilic [HP]) in different dissolution media, with and without enzymes, were established. Also investigated was the influence of a treatment of the
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