M5057
MES sodium salt
BioReagent, ≥99% (titration), crystalline
Sinónimos:
Ácido 2-(N-morfolino)etanosulfónico sodium salt, Ácido 4-morfolinoetanosulfónico sodium salt
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Fórmula empírica (notación de Hill):
C6H12NNaO4S
Número CAS:
Peso molecular:
217.22
NACRES:
NA.25
PubChem Substance ID:
eCl@ss:
32129211
UNSPSC Code:
12161700
EC Number:
275-203-2
MDL number:
Beilstein/REAXYS Number:
3765682
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Permítanos ayudarleInChI key
IRHWMYKYLWNHTL-UHFFFAOYSA-M
InChI
1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
SMILES string
[Na+].[O-]S(=O)(=O)CCN1CCOCC1
product line
BioReagent
assay
≥99% (titration)
form
crystalline
pH
8.9-10.1 (1% in water)
Quality Level
useful pH range
5.5-6.7
pKa
6.1
solubility
water: 0.5 g/mL, clear, colorless
application(s)
diagnostic assay manufacturing
storage temp.
room temp
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General description
MES is one of the so-called "Good" buffers developed for biological applications. This product is tested for trace ions. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Application
MES sodium salt has been used:
- To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA
- To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization
Preparation Note
MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions should be stable at 2-8°C for months. Sterilization should be by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.
Other Notes
Easily compare specifications for MES sodium salt products with the MES sodium salt specification table.
Clase de almacenamiento
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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