P5747
Anti-Phosphoserine antibody, Mouse Monoclonal
clone PSR-45, purified from hybridoma cell culture
Sinónimos:
Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine
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NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
PSR-45, monoclonal
Application:
ARR, DB, ELISA (i), WB
Technique(s):
dot blot: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, microarray: suitable, western blot: 2.5-5.0 μg/mL using total rat brain extract.
Citations:
36
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Permítanos ayudarlebiological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PSR-45, monoclonal
form
buffered aqueous solution
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
dot blot: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, microarray: suitable, western blot: 2.5-5.0 μg/mL using total rat brain extract.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
The antibody has been used for the detection of some phosphoserine containing proteins using immunoblotting and dot blotting.
Biochem/physiol Actions
By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.
Protein phosphorylation and dephosphorylation are basic signaling mechanisms that modify protein function in eukaryotic cells. Phosphorylation is a rare posttranslational event in normal tissues, however, the abundance of phosphorylated cellular proteins increases several folds following various activation processes. The main amino acids that are phosphorylated are tyrosine, serine, or threonine (pTyr/pSer/pThr), each having specific kinases that phosphorylate them and specific phosphatases that dephosphorylate them. Mono and polyclonal antibodies directed against phosphorylated residues were generated and found useful as analytical and preparative tools by enabling the identification, quantification, and immunoaffinity isolation of phosphorylated cellular proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Clase de almacenamiento
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Matteo Fornai et al.
The Journal of pharmacology and experimental therapeutics, 356(2), 434-444 (2015-11-20)
Parkinson's disease is frequently associated with gastrointestinal symptoms, mostly represented by constipation and defecatory dysfunctions. This study examined the impact of central dopaminergic denervation, induced by injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle, on distal colonic excitatory cholinergic
An integrated assessment of histopathological changes of the enteric neuromuscular compartment in experimental colitis
Ippolito C, et al.
Journal of Cellular and Molecular Medicine, 19(2), 485-500 (2015)
M Zhao et al.
FEBS letters, 509(3), 405-412 (2001-12-26)
B-cell leukemia/lymphoma 2 (Bcl-2) blocks oxidant-induced apoptosis at least partly by stabilizing lysosomes. Here we report that phosphorylation of Bcl-2 may be required for these protective effects. J774 cells overexpressing wild-type Bcl-2 resist oxidant-induced lysosomal leak as well as apoptosis
P R Murphy et al.
Endocrinology, 142(1), 81-88 (2001-01-06)
We recently reported that the rat Nb2 T lymphoma cells expressed messenger RNAs (mRNAs) encoding both fibroblast growth factor-2 (FGF-2) and the FGF receptor, suggesting possible paracrine and/or autocrine roles for FGF-2 in lymphoma cell function. We have also shown
Mechanisms of specificity in protein phosphorylation
Ubersax JA and Ferrell Jr JE
Nature Reviews in Molecular and Cell Biology, 8(7), 530-530 (2007)
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