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Merck

T6567

Trypsin Protease

Hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, from Porcine pancreas

Sinónimos:

Porcine Trypsin, Trypsin for Mass Spectropetry

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UNSPSC Code:
12352204
NACRES:
NA.56
EC Number:
232-650-8
MDL number:
MFCD01775455
Biological source:
Porcine pancreas

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Nombre del producto

Tripsina from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated

biological source

Porcine pancreas

product line

BioReagent

solubility

1 mM HCl: soluble 1 mg/mL, clear, colorless

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Categorías relacionadas

Application

La tripsina del páncreas porcino se utiliza para las siguientes aplicaciones:
  • Digestión proteica en gel y análisis mediante espectrometría de masas con MALDI-TOF
  • Análisis mediante espectrometría de masas de ionización por electrospray (ESI-MS)
  • Perfilado del proteoma de superficie de L. plantarum
  • Filtración en gel, ultracentrifugación y microscopia electrónica de sombras rotatorias
  • Espectrometría de masas

Biochem/physiol Actions

La tripsina se utiliza habitualmente en la investigación proteómica para la cartografía de péptidos y el trabajo de secuenciación de proteínas, debido a su escisión muy específica que produce un número limitado de péptidos tripsínicos. Hidroliza los enlaces peptídicos específicamente en el lado carboxilo de los restos de arginina y lisina. La enzima exhibe también actividades esterasa y amidasa. La tripsina actúa como una herramienta de cultivo celular. Se utiliza para hidrolizar proteínas alergénicas y producir leche hipoalergénica en la industria.

General description

La tripsina es una proteasa de serina que se utiliza generalmente en bioquímica y biología como un reactivo enzimático importante. Este método produce un producto de tripsina muy purificado adecuado para la investigación en proteómica. Grado proteómica ideal para su uso tanto en soluciones como en hidrolizados de tripsina en gel. La tripsina, una proteasa de serina, está presente en el sistema digestivo de varios vertebrados.

Other Notes

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
SLCL1499
SLCG2226
SLCK2811
SLCJ6728
SLBZ2416

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What can I use to solubilize this Product T6567, Trypsin from porcine pancreas?

    This product is soluble in 1mM HCL.

  6. What is the package size of Product T6567, Trypsin from porcine pancreas?

    Each vial content 20 ug of the product.

  7. Is Product T6567, Trypsin from porcine pancreas, TPCK treated?

    The product has been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized, resulting in convenient use and highly specific cleavage. This information is on the product page under application.

  8. How can we get Trypsin sequence information for Product T6567, Trypsin from porcine pancreas?

    If you are looking for trypsin sequence information, you have to go to the NCBI Protein Data Bank.

  9. How can we digest protein using Product T6567, Trypsin from porcine pancreas?

    As per Sigma R and D the information is as follows: In order to efficiently digest a protein with trypsin, it must be denatured and the disulfide bonds modified by reduction and alkylation, or at least reduced. Many intact proteins are highly resistant to digestion with trypsin. If you do not want to reduce and alkylate, you can then just boil the protein with 5 mM DTT or 20 mM 2ME for 10 minutes, and then quickly cool on ice to denature the protein. This may result in a precipitate, but the trypsin will still digest the protein and it will clear within an hour or two. You can also dissolve the protein in 6 M guanidine-HCl or 8 M urea. Reduce and alkylate using the PROT-RA kit or other suitable method. Then they would have to dilute the solution to less than 2 M of either denaturant and then add the trypsin. This is the method that we use routinely in the lab.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Pauline Floch et al.
Gut pathogens, 6, 20-20 (2014-07-06)
A gamma-glutamyl transpeptidase (GGT) is produced by up to 31% of strains of Campylobacter jejuni isolates. C. jejuni GGT is close to Helicobacter pylori GGT suggesting a conserved activity but unlike the latter, C. jejuni GGT has not been studied
Yusuke Murasawa et al.
The Journal of biological chemistry, 288(40), 29170-29181 (2013-08-22)
Versican G1 domain-containing fragments (VG1Fs) have been identified in extracts from the dermis in which hyaluronan (HA)-versican-fibrillin complexes are found. However, the molecular assembly of VG1Fs in the HA-versican-microfibril macrocomplex has not yet been elucidated. Here, we clarify the role
Masayuki Shimoda et al.
Cell transplantation, 21(2-3), 465-471 (2012-07-17)
Porcine islets are considered to be a promising resource for xenotransplantation. However, it is difficult to isolate porcine islets because of the marked fragility and rapid dissociation. Endogenous trypsin is one of the main factors to damage islets during the
Hui Zheng et al.
Molecules (Basel, Switzerland), 18(11), 13425-13433 (2013-11-02)
A novel chemoenzymatic one-pot multicomponent synthesis of thiazole derivatives was developed. A series of thiazole derivatives were synthesized with high yields up to 94% under mild enzyme-catalyzed conditions. The blank and control experiments reveal that trypsin from porcine pancreas (PPT)
Maciej Suski et al.
Biochimica et biophysica acta, 1834(12), 2463-2469 (2013-08-31)
Excessive action of angiotensin II on mitochondria has been shown to play an important role in mitochondrial dysfunction, a common feature of atherogenesis and kidney injury. Angiotensin-(1-7)/Mas receptor axis constitutes a countermeasure to the detrimental effects of angiotensin II on
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