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Merck

T7816

Monoclonal Anti-β-Tubulin I antibody produced in mouse

clone SAP.4G5, ascites fluid

Sinónimos:

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02095890

Documentos clave

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Nombre del producto

Monoclonal Anti-β-Tubulin I antibody produced in mouse, clone SAP.4G5, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SAP.4G5, monoclonal

mol wt

antigen ~55 kDa

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:20,000 using cultured chicken fibroblasts

isotype

IgG1

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

Application

Monoclonal anti-β-tubulin I antibody (diluted 1: 4,000) can be used as a primary antibody in immunoprecipitation and immunoblotting. It can also be used in western blot analysis and retroviral infection.

Biochem/physiol Actions

Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulin a 100kD protein is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin I antibody can be used in ELISA to localize β tubulin isotype I and can be used in immunoblotting to localize cultured human and animal cells. Mouse anti-β-tubulin I antibody reacts specifically with an epitope found in the c-terminal sequence of β tubulin of human, rat, dog, bovine, hamster, mouse, chicken and xenopus

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.

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Clase de almacenamiento

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
068M4850V
22180407
058M4754V
087M4877V
025M4776V

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A A Atwood et al.
Oncogene, 29(45), 6004-6015 (2010-09-08)
Overexpression of Ras(V12) in MCF10A cells, an immortalized mammary epithelial cell line, leads to transformation of these cells. We demonstrate that this is accompanied by degradation of C/EBPbeta1. C/EBPbeta is a transcription factor in which three protein isoforms exist because
Geza Gemes et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(10), 3536-3549 (2011-03-11)
Painful nerve injury disrupts levels of cytoplasmic and stored Ca(2+) in sensory neurons. Since influx of Ca(2+) may occur through store-operated Ca(2+) entry (SOCE) as well as voltage- and ligand-activated pathways, we sought confirmation of SOCE in sensory neurons from
Yosef Buganim et al.
Cancer research, 66(22), 10750-10759 (2006-11-17)
Mutations in p53 are ubiquitous in human tumors. Some p53 mutations not only result in loss of wild-type (WT) activity but also grant additional functions, termed "gain of function." In this study, we explore how the status of p53 affects
Nathan D Camp et al.
The Journal of biological chemistry, 287(9), 6539-6550 (2012-01-05)
WTX is a tumor suppressor protein that is lost or mutated in up to 30% of cases of Wilms tumor. Among its known functions, WTX interacts with the β-transducin repeat containing family of ubiquitin ligase adaptors and promotes the ubiquitination
Ning Zhao et al.
Circulation research, 116(1), 23-34 (2014-10-18)
MicroRNA miR145 has been implicated in vascular smooth muscle cell differentiation, but its mechanisms of action and downstream targets have not been fully defined. Here, we sought to explore and define the mechanisms of miR145 function in smooth muscle cells.
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