Extracellular Vesicle Preparation

Extracellular vesicles (EVs), such as exosomes, are fascinating particles that can deliver proteins, lipids, and RNAs to distant target cells. Critical to understanding EV function is reliable enrichment of pure fractions. Many investigators use ultracentrifugation, which is lengthy and labor intensive. However, size exclusion, using low-MWCO ultrafiltration membranes, is becoming more and more widely used and cited in literature.

Learn how to ensure your EV preparations are reproducible and accurate with:

• Rapid, centrifugal ultrafilter-based method using Amicon^® Ultra filters
• Equivalent yield/quality to ultracentrifuge preparations
• Better yield/quality than polymeric precipitation-based techniques

Read EV Preparation Protocol

Diverse Methods Used for Exosome and Extracellular Vesicle Preparation

Given the many methods available for EV preparation, it is useful to consider the methods in light of the scientific question you are asking and downstream analytical techniques. This table shows a snapshot of EV preparation methods and some of their advantages and disadvantages. We hope it will help you better plan your extracellular vesicle and exosome research!