40718
Diethyl pyrocarbonate
96% (NT)
동의어(들):
DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
O(COOC2H5)2
CAS 번호:
Molecular Weight:
162.14
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
216-542-8
Beilstein/REAXYS Number:
637031
MDL number:
Assay:
96% (NT)
Form:
liquid
InChI
1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
SMILES string
CCOC(=O)OC(=O)OCC
InChI key
FFYPMLJYZAEMQB-UHFFFAOYSA-N
assay
96% (NT)
form
liquid
color
APHA: ≤20
Quality Level
bp
93-94 °C/18 mmHg (lit.)
solubility
95% ethanol: 4.5 g/10 mL, clear, colorless
density
1.121 g/mL at 20 °C, 1.101 g/mL at 25 °C (lit.)
functional group
carbonate
shipped in
wet ice
storage temp.
2-8°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
관련 카테고리
General description
Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.
Application
Diethyl pyrocarbonate may be used in the following studies:
- For the modification of histidyl residues in proteins.
- As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
- For the modification of linear and supercoiled plasmid DNAs.
- As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
- For cabethoxylation of histidine residues of actin.
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
Biochem/physiol Actions
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
156.2 °F - closed cup
flash_point_c
69 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Modification of histidyl residues in proteins by diethylpyrocarbonate.
E W Miles
Methods in enzymology, 47, 431-442 (1977-01-01)
Selective carbethoxylation of the histidine residues of actin by diethylpyrocarbonate.
G Hegyi et al.
European journal of biochemistry, 44(1), 7-12 (1974-05-02)
W Herr
Proceedings of the National Academy of Sciences of the United States of America, 82(23), 8009-8013 (1985-12-01)
Purine residues located within regions of DNA that have the potential to form left-handed Z-helical structures are modified preferentially by diethyl pyrocarbonate; this hyperreactivity is dependent on the degree of negative superhelicity of the circular DNA molecules. As negative superhelical
Effects of diethyl pyrocarbonate and methyl methanesulfonate on nucleic acids and nucleases.
I Fedorcsák et al.
Acta chemica Scandinavica, 20(1), 107-112 (1966-01-01)
A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues.
F Solymosy et al.
European journal of biochemistry, 5(4), 520-527 (1968-09-24)
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.