Anti-Phosphatidylserine Antibody, clone 1H6

Significance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.

Phosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. PS is most concentrated in the brain, as PS makes up about 10% of the lipid content of neurons. Neural PS is thought to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. One of the key features of apoptosis is the expresion of PS on the outer cell membrane. PS is usually found on the inner side of the plasma membrane, but during early stage apoptosis, the PS is translocated to the outer membrane. FITC-labeled annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays.

none given

Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol

Anti-Phosphatidylserine Antibody, clone 1H6 is an antibody against Phosphatidylserine for use in FC, IH.

Flow Cytometry: 0.2-2 μg/mL of a previous lot detected cell surface phosphatidylserine on Jurkat cells treated with Fas Ligand vesicles (Catalog # 01-210). See page two.

Immunohistochemistry: This antibody has been reported by an independent laboratory to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone (Mandinov, L., 2003).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Recognizes phosphatidylserine (PS) in cell membranes

Format: Purified

Protein G Purified

Purified IgG supplied in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4 before the addition of glycerol to 30%. Liquid at -20°C.

Stable for 1 year at from date of receipt.

Control
Brain tissue

routinely evaluated by flow cytometry on Jurkat cells treated with Fas Ligand vesicles (Catalog #01-210)

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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