Anti-NR2B Antibody

Glutamate receptor ionotropic, NMDA 2B (UniProt: Q01097; also known as GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B) is encoded by the Grin2b gene (Gene ID: 14812) in mouse. NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B or NR2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA). Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer′s disease, epilepsy and ischemic neuronal cell death.

Target molecular weight ~165 kDa observed; 165.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal, cytoplasmic domain of mouse NR2B.

Anti-NR2B, Cat. No. 06-600, is a rabbit polyclonal antibody that detects NR2B and is tested for use in Western Blotting.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases

This rabbit polyclonal antibody specifically detects NR2B. It targets an epitope within 20 amino acids from the C-terminal, cytoplasmic domain.

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Control
Mouse brain tissue

Evaluated by Western Blotting in rat brain microsomal preparation. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NR2B in rat brain microsomal preparation.

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