Anti-Histone H2A (acidic patch) Antibody

14 kDa

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

KLH-conjugated, synthetic peptide corresponding to amino acids 88-97 (IRNDEELNKL-C) of human Histone H2A.

Anti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB.

Immunofluorescence:
Recommend Anti-phospho-Histone H2A.X (Ser139), clone JBW301, catalog #05-636.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Broad species cross-reactivity is expected.

Recognizes Histone H2A, Mr 14 kDa.

Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide. Frozen solution.

Unpurified

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa cell extract.

Routinely evaluated by western blot on acid extracts from HeLa cell lysate.

Western Blot Analysis:
1:1000-1:5000 dilutions of this lot detected Histone H2A (acidic patch) in acid extracts from HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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