Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24)

Arginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA).

Varies. Antibody detects proteins containing asymmetrical dimethylated arginines.

KLH-conjugated, synthetic peptide containing asymmetric dimethyl-arginine-glycine repeats (KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G in which R* denotes asymmetric dimethylation of arginine).

Detect dimethyl-Arginine with Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) (Rabbit Polyclonal Antibody), that has been shown to work in WB.

Reactivity with other species has not been confirmed.

Recognizes proteins that contain arginines that are asymmetrically dimethylated including p300, p110, p75 and Sam68. Specificity for asymmetrically dimethylated arginine was determined by ELISA with an unmethylated ‘backbone’ peptide or an identical peptide containing symmetrically dimethylated arginines.

Rabbit antiserum diluted in storage buffer (PBS, 0.035% sodium azide and 30% glycerol). Store at -20°C.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Routinely evaluated by Western Blot HeLa nuclear extract and p110, p75, and Sam68 in RIPA lysates from Jukat cells.

Western Blot Analysis:
A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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