Anti-NR2A Antibody

NR2 or glutamate receptor, ionotropic, N-methyl D-aspartate 2 (NMDA2; NMDAR2) along with the NR1 subunit forms a heterodimer that make up the NMDA receptor. By alternative splicing there are various isoforms of NR2 classified as NR2A through D. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. The NR1 subunit binds glycine while the NR2 subunit binds glutamine for activation. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

~170 kDa

6His-tagged fusion protein corresponding to amino acids 1265-1464 of mouse NMDA receptor 2A (NR2A).

Detect NR2A using this Anti-NR2A Antibody validated for use in IP & WB.

Immunoprecipitation:
4 µg of a previous lot immuno-precipitated NR2A from rat brain hippocampus membranes and rat brain microsomal preparations.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Recognizes NR2A, MW ~170 kDa.

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Stable for 1 year at -20ºC from date of receipt.

Control
Brain tissue.

Evaluated by western blot in rat brain microsomal preparations (Catalog # 12-144).

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected NR2A in rat brain microsomal preparations (Catalog # 12-144).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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