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Merck

1.12018

Albumin fraction V

(from bovine serum) for biochemistry

동의어(들):

Albumin fraction V, Bovine serum albumin, Serum albumin

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
MDL number:
NACRES:
NA.21
EC Index Number:
292-322-5
UNSPSC Code:
12352202
Biological source:
bovine
Form:
solid
Technique(s):
immunoassay: suitable, western blot: suitable
Concentration:
≥97% protein (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))
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biological source

bovine

form

solid

concentration

≥97% protein (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))

technique(s)

immunoassay: suitable, western blot: suitable

pH

6.8-7.2 (20 °C, 1 g/L in H2O)

solubility

1 g/L

bulk density

150 kg/m3

storage temp.

2-8°C

General description

Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each. This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.

Application

Albumin fraction V has been used:
  • as a component of perfusion buffer for perfusion protocol for rodent organ perfusion
  • as a blocking agent in western blot analysis
  • as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the chief component of cell culture media and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids. 

Analysis Note

Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

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Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter

문서

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

국제 무역 품목 번호

SKUGTIN
1.12018.010004022536138367
1.12018.002504022536138350

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