Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotrans-mission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that over 95% of all phosphorylation events occur on serine and threonine residues.

Varies

Epitope: phosphoserine

Phosphoserine conjugated to KLH.

Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate is an antibody against Phosphoserine for use in WB.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Recognizes proteins containing phosphoserine residues, regardless of origin.

100 μL of protein G purified mouse IgG1 in PBS containing preservative and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:4000 in 10 mL blocking buffer. Liquid.

Protein G Purified

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

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