17-10400

Anti-phospho-MLKL (Ser358) Antibody Set

Name: Anti-phospho-MLKL (Ser358) Antibody Set
Brand: MM

from rabbit, purified by affinity chromatography

동의어(들):

Mixed lineage kinase domain-like protein, MLKL

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Clone:

polyclonal

Species reactivity:

human

Application:

Citations:

주요 문서

모든 문서 보기

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도움 문의

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

UniProt accession no.

Q8NB16

shipped in

wet ice

target post-translational modification

phosphorylation (pSer358)

Quality Level

100

Gene Information

human ... MLKL(197259)

General description

Mixed lineage kinase domain-like protein (MLKL) is required for the execution of programmed necrosis. MLKL is a cell-permeable acrylamide compound that inhibits human, but not murine, MLKL adaptor function via covalent modification of Cys86. MLKL prevents the MLKL-RIP1-RIP3 necrosome complex from interacting with further downstream effectors.

This phospho-MLKL (Ser358) antibody set contains one vial of phospho-MLKL (Ser358) antibody and one vial of MLKL “Total” antibody.

RECOMMENDED USAGE:
It is recommended to first perform immunoprecipitation on your sample using the non-phosphorylated MLKL antibody for “total” MLKL protein isolation (see protocol section on page 2 of the Certificate of Analysis); then use the immunoprecipitated sample to detect the phosphorylated protein using the phospho-MLKL (Ser358) antibody via western blot.

~54 kDa

Immunogen

Epitope: Ser358

Linear peptide corresponding to the Protein Kinase Domain of phospho-MLKL (Ser358).

Application

Western Blot Analysis:
Untreated HT-29 cell lysates or lysates from HT-29 cells treated with Cycloheximide,Z-VAD-FMK and TNF alpha were first immunoprecipitated using the Anti-MLKL “total” antibody (1:1000 dilution) to isolate the MLKL protein.
The isolated “total” MLKL protein sample was then probed with Anti-phospho-MLKL (Ser358) (1:1000 dilution).

Anti-phospho-MLKL (Ser358) Antibody Set is an antibody set against phospho-MLKL (Ser358) for use in WB.

Biochem/physiol Actions

When used together and under the “Recommended Usage” instructions, this antibody set will detect MLKL phosphorylated at Ser358.

Physical form

100 µL of Anti-phospho-MLKL (Ser358) purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

100 µg (concentration: 1 mg/mL) of Anti- MLKL purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

Analysis Note

Anti- MLKL (Total Antibody): Evaluated by Immunoprecipitation on HT-29 cell lysates.

Anti-phospho-MLKL (Ser358) Antibody: Evaluated by Dot Blot on phosphorylated and non-phosphorylated MLKL peptides.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

One vial of phospho-MLKL (Ser358) antibody

One vial of MLKL “Total” antibody

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저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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국제 무역 품목 번호

SKU	GTIN
17-10400	04053252934599

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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Anti-phospho-MLKL (Ser358) Antibody Set

from rabbit, purified by affinity chromatography

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

biological source

antibody form

antibody product type

clone

purified by

species reactivity

technique(s)

UniProt accession no.

shipped in

target post-translational modification

Quality Level

Gene Information

설명

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Analysis Note

Other Notes

안전성 정보

저장 등급

flash_point_f

flash_point_c

문서

시험 성적서(COA)

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