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Merck

AB144P

Anti-Choline Acetyltransferase Antibody

CHEMICON®, goat polyclonal

동의어(들):

Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken
Application:
ICC, IHC (p), WB
Citations:
1310
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제품 이름

Anti-Choline Acetyltransferase Antibody, Chemicon®, from goat

biological source

goat

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

chicken ... Chat(395314)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

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General description

70/74 kDa
Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.

Immunogen

Human placental enzyme.

Application

Anti-Choline Acetyltransferase Antibody has been used in:
  • immunohistochemical staining
  • western blotting
  • immunocytochemistry

Immunohistochemistry:
A previous lot of this antibody was used at 1:100.

Optimal working dilutions and protocols must be determined by end user.

Biochem/physiol Actions

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.
Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

Physical form

Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

Analysis Note

Control
Human placenta lysates, rat forebrain/rostral hypothalamus.
Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

Other Notes

Research Category:
Neuroscience

Research Sub Category:
Neurotransmitters & Receptors

Neuronal & Glial Markers

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The human choline acetyltransferase gene encodes two proteins
Grosman DD, et al.
Journal of Neurochemistry (1995)
Identified motoneurons involved in sexual and eliminative functions in the rat are powerfully excited by vasopressin and tachykinins.
Ogier, R; Tribollet, E; Suarez, P; Raggenbass, M
The Journal of Neuroscience null
Sigma-1 Receptor Plays a Negative Modulation on N-type Calcium Channel
Zhang K, et al.
Frontiers in Pharmacology, 302-302 (2017)
Plasticity of motor network and function in the absence of corticospinal projection
Han Q, et al.
Experimental Neurology, 194-208 (2015)
Cholinergic modulation of synaptic transmission and postsynaptic excitability in the rat gracilis dorsal column nucleus.
Fernandez de Sevilla, D; Rodrigo-Angulo, M; Nu?ez, A; Bu?o, W
The Journal of Neuroscience null

문서

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

구조, 클래스, 면역글로불린의 정상 범위에 관한 기술 정보 등 항체 작업의 기본 원칙에 대해 알아보십시오.

관련 콘텐츠

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국제 무역 품목 번호

SKUGTIN
AB144P08436037123283
AG22004053252274930
AB144P-1ML04053252468797
AB144P-200UL04053252268984

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