Anti-Metabotropic Glutamate Receptor 5 Antibody, pain

Metabotropic glutamate receptor 5 or mGluR5 (UniProt Q3UVX5; also known as G protein-coupled receptor GRM5, G protein coupled receptor family C, group 1, member E) is encoded by the Grm5 (also known as Gprc1e, Mglur5, AI850523, 6430542K11Rik) gene in murine species (Gene ID 108071). The metabotropic glutamate receptors belong to a family of G protein-coupled receptors that have been divided into 3 groups based on their sequence homology, putative signal transduction mechanisms, and pharmacologic properties. mGluR1 and mGluR5 constitute Group I metabotropic glutamate receptors. mGluR5 is found on postsynaptic terminals of neurons and microglia. mGluR5 signals via Gq/G11 coupling to activate phospholipase C, resulting in calcium mobilization and activation of protein kinase C (PKC). In addition, mGluR5 is also reported to mediate signaling involving Src-family protein tyrosine kinase in a G protein-independent manner.

~150 kDa observed. Uncharacterized band(s) may appear in some lysates.

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of mouse Metabotropic Glutamate Receptor 5.

Anti-Metabotropic Glutamate Receptor 5 Antibody, pain is an antibody against Metabotropic Glutamate Receptor 5 for use in IH & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western Blotting Analysis: 0.1-0.5 μg/mL using ECL on rat brain microsomal preparation. Reacts with a band of ~132 kDa.
Immunohistochemistry: 0.1-0.2 μg/mL on rat brain and mouse brain sections fixed with 4% paraformaldehyde containing 15% saturated picric acid in 0.1M PB, pH 7.4.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Metabotropic Glutamate Receptor 5 in mouse hindbrain and rat colon tissue.

This antibody will not detect Isoform 2.

ImmunoAffinity Purified

Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Brain tissue

Evaluated by Western Blotting in microsomal preparation of rat brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Metabotropic Glutamate Receptor 5 in 10 µg of microsomal preparation of rat brain tissue lysate.

Replaces: AB1596; 06-451

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