Anti-β-Catenin Antibody

β-catenin is the founding member of the β -catenin family. This protein is comprised of 12 ARM repeats and is considered to have dual function depending on its localization. Cytoplasmic β -catenin is a critical component of E-cadherin/catening adhesion complexes and is involved in regulating cadherin-dependent cell-cell interactions.When localized to the nucleus, β -catenin serves a regulatory role by binding with transcription factors from the TCF/LEF family, activating transcription. Expression of β -catenin has been reported in a variety of hair follicle cells such as cells found in the inner and outer portions of the root sheaths, and the basal and peripheral matrix cells. It has also been observed in colon tissue. Defects in expression have been linked to the development of several tumors and cancers such as; colorectal cancer (CRC), medulloblastoma (MDB), ovarian cancer (OC), and pilomatrixoma (PTR).

~86kDa observed.

Epitope: Consensus GSK3 phosphorylation site of human β-Catenin

KLH-conjugated linear peptide corresponding to the consensus GSK3 phosphorylation site of human β-Catenin.

Anti-β-Catenin Antibody is a Rabbit Polyclonal Antibody for detection of β-Catenin also known as catenin (cadherin-associated protein) beta 1 (88kD) & has been validated in WB, ICC & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated β-Catenin from A431 lysate.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected β-Catenin in NIH/3T3 and A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Antibody recognizes β-Catenin at the N-terminus.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
A431 cell lysate

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected β-Catenin on 10 µg of A431 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1002

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