CC054
Human Collagen Type III
from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation
동의어(들):
Collagen III from human placenta, alpha 1 Type III Collagen
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
human
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
1 mg/mL
Assay:
90% (Human collagen type III, SDS-PAGE)
제품 이름
Human Collagen Type III, 1 mg/mL, solution, Bioreagent, suitable for cell culture
biological source
human
assay
90% (Human collagen type III, SDS-PAGE)
form
solution
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
impurities
<1% Human collagen types II, IV-VI, and non-collagen proteins, <10% Human collagen type I
input
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
solubility
water: soluble at 20 °C
NCBI accession no.
UniProt accession no.
binding specificity
Peptide Source: Fibrinogen
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... COL3A1(1281)
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General description
The COL3A1 gene mapped to human chromosome 2q32.2, encodes the alpha 1 chain of type III collagen. Type III collagen is an extracellular matrix protein containing a long triple-helical domain made of three alpha 1 chains. Collagen III is a major structural component in hollow organs such as large blood vessels, the uterus and the bowel. Human type III collagen is purified by serial salt precipitations, alcohol precipitation, and DEAE chromatography of an acetic acid extraction of human placenta, following mild pepsin digestion. Product Source: Human placenta, negative for HbsAG, HCV and HIV 1 & 2 antibodies.
Application
Human Collagen Type III has been used:
- as an extracellular matrix (ECM) protein in microarray analysis, to coat nitrocellulose film slides for probing and detection experiments
- to coat microfluidic migration devices and to study the impact of extracellular matrix (ECM) on cell migration
- as a positive control in Gpr56 PRESTO-Tango assay and GTP-Rho pull-down assay, to evaluate its effects on cellular signaling and interactions
Biochem/physiol Actions
Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. The type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen tissue strengthening function
Physical form
Liquid in 0.5 M acetic acid, pH 2.5.
Preparation Note
Maintain at -20ºC in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Retention of native structure was confirmed by ability to form microfibrils.
Other Notes
Composition: [a1(III)]3, native triple helix. Ability to form native helical structure verified by ORD measurement, competence in microfibril formation and reactivity with anti-collagen type-specific monoclonal antibodies
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Physicochemical characterization and molecular organization of the collagen A and B chains.
R K Rhodes et al.
Biochemistry, 17(17), 3442-3448 (1978-08-22)
Guido Carpino et al.
Clinical proteomics, 16, 37-37 (2019-11-07)
Intrahepatic cholangiocarcinoma (iCCA) is a malignancy that arises from the intrahepatic biliary tree, showing high mortality rates due to its late clinical presentation and limited treatment options. iCCA is characterized by a dense, reactive desmoplastic stroma marked by a dramatic
Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
Sage, H and Bornstein, P
Biochemistry, 18, 3815-3822 (1979)
R W Glanville et al.
European journal of biochemistry, 95(2), 383-389 (1979-04-02)
Native type IV collagen was isolated from human placenta using pepsin solubilisation followed by fractional salt precipitation and chromatogarphic purification. The native preparation was characterised using amino acid analyses, disc gel electrophoresis, segment-long-spacing crystallites and immunological methods. Two component alpha
프로토콜
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| CC054 | 04053252409110 |
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