biological source
human
assay
>98% (SDS-PAGE and HPLC)
specific activity
≥2 x 10(E7) U/mg
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)
input
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Gene Information
human ... IFNG(3458)
General description
Interferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
Product Source: Human, expressed in E.coli
Application
For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.
Physical form
Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.
Preparation Note
The lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.
Analysis Note
Specific Activity: Human IFN-gamma is fully biologically active when compared to standards.The ED(50) as determined in a viral resistance assay is ≤ 0.05 ng/mL
Other Notes
Replaces: IF3
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
11 - Combustible Solids
wgk
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Hanna Galganska et al.
Cellular and molecular life sciences : CMLS, 78(24), 8229-8242 (2021-11-07)
Mitogen-activated protein kinase (MAPK) signalling pathways are crucial for developmental processes, oncogenesis, and inflammation, including the production of proinflammatory cytokines caused by reactive oxygen species and upon severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. There are no drugs that
Audrey Xavier et al.
Cell reports, 31(7), 107667-107667 (2020-05-21)
Human guanylate binding protein 1 (hGBP1) belongs to the dynamin superfamily of GTPases and conveys host defense against intracellular bacteria and parasites. During infection, hGBP1 is recruited to pathogen-containing vacuoles, such as Chlamydia trachomatis inclusions, restricts pathogenic growth, and induces
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| IF002 | 04053252284038 |
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