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Merck

ISEQ00010

Immobilon® -PSQ PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.2 µm pore size, transfer membrane for Western blotting

동의어(들):

Western blotting membrane, blotting membrane, transfer membrane

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.23
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, radioactive
Pore size:
0.2 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
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제품 이름

Immobilon®-PSQ PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Level

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, radioactive

shipped in

ambient

General description

The Immobilon®-PSQ membrane is a 0.2 μm microporous membrane made up of polyvinylidene fluoride (PVDF). It has been developed to maximize protein binding. Immobilon®-PSQ membrane is recommended for direct protein sequencing and immunodetection following electroblotting of protein from electrophoresis gels (or dot binding of purified protein samples), especially for proteins with molecular weights less than 20,000. This membrane is compatible with tank and semi-dry electroblotting systems.

Application

Immobilon®-PSQ PVDF Membrane has been used in western blot.

Features and Benefits

Immobilon®-PSQ Membrane:
  • 0.2 μm pore size and a large internal structure
  • Higher protein adsorption and sequencing yields than other membranes
  • Prevents blow-through of low molecular weight proteins

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany


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저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 분석 증명서(COA)를 검색하십시오. 로트 및 배치 번호는 제품 라벨의 ‘Lot’ 또는 ‘Batch’라는 단어 뒤에 기재되어 있습니다.

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문서 라이브러리 방문


프로토콜

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.


Shujie Wang et al.
Infection and immunity, 88(4) (2020-01-15)
Streptococcus suis serotype 2 is an important bacterial pathogen of swine and is also an emerging zoonotic agent that may be harmful to human health. Although the virulence genes of S. suis have been extensively studied, the mechanisms by which
Bing Yan et al.
Theranostics, 10(15), 6728-6742 (2020-06-20)
Rational: Ischemic injury of the skeletal muscle remains a serious clinical problem and currently there is no effective therapy. The aim of the present study is to determine whether human umbilical cord mesenchymal stem cells- derived exosomes (UMSC-Exo) could repair
Daisy Y Shu et al.
Experimental eye research, 206, 108546-108546 (2021-03-29)
Transforming growth factor beta (TGFβ) and bone morphogenetic protein (BMP) signaling play opposing roles in epithelial-mesenchymal transition (EMT) of lens epithelial cells, a cellular process integral to the pathogenesis of fibrotic cataract. We previously showed that BMP-7-induced Smad1/5 signaling blocks



국제 무역 품목 번호

SKUGTIN
ISEQ0001004053252334306