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Merck

MAB1281

Anti-Nuclei Antibody, clone 235-1

clone 235-1, Chemicon®, from mouse

동의어(들):

HuNu

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크기 선택


제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
235-1, monoclonal
Species reactivity:
human
Application:
ICC, IHC, IP
Citations:
387
기술 서비스
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도움 문의

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

235-1, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable

input

sample type neural stem cell(s)

isotype

IgG1

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

General description

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Anti-nuclei antibodies are useful cytological and histological markers for identifying human cells in xenograft models.

Application

Anti-Nuclei Antibody, clone 235-1 has been used in: Immunohistochemistry (1:200); Immunolabeling for human nuclei (HuNu) (1:300); Histological and immuno-fluorescent analysis of submandibular glands

Biochem/physiol Actions

Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.

Physical form

Format: Purified
Hybridoma supernatant. Liquid in PBS. Contains no preservative.
Protein A purified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
All human cell types

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Long-term hibernation of human fetal striatal tissue does not adversely affect its differentiation in vitro or graft survival: implications for clinical trials in Huntington's disease.
Hurelbrink, Carrie B, et al.
Cell Transplantation, 12, 687-695 (2003)
Improving the survival of human CNS precursor-derived neurons after transplantation.
Le Belle, J E, et al.
Journal of Neuroscience Research, 76, 174-183 (2004)
Infusion of human umbilical cord blood cells in a rat model of stroke dose-dependently rescues behavioral deficits and reduces infarct volume.
Vendrame, Martina, et al.
Stroke, 35, 2390-2395 (2004)
Transplantation of cultured neural cells from human fetuses into the brain of rats exposed to acute hypoxia.
Aleksandrova, M A, et al.
Bulletin of experimental biology and medicine, 137, 262-265 (2004)
Human umbilical cord blood cells express neural antigens after transplantation into the developing rat brain.
Zigova, Tanja, et al.
Cell Transplantation, 11, 265-274 (2002)

문서

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.

Immunofluorescence uses antibody-conjugated fluorescent molecules for protein localization, modification confirmation, and protein complex visualization.

모두 보기

프로토콜

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

국제 무역 품목 번호

SKUGTIN
MAB128104053252317378

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