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Merck

MAB1637

Anti-β-Tubulin III Antibody

CHEMICON®, mouse monoclonal, TU-20

동의어(들):

Tubulin beta-III, tubulin, beta 3, tubulin, beta 4

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
TU-20 (Similar to TUJ1), monoclonal
Species reactivity:
rat, mouse, avian, human, bovine, monkey, pig, sheep
Application:
ELISA, ICC, IHC (p), IP, WB
Citations:
421

주요 문서

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제품 이름

Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1), ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TU-20 (Similar to TUJ1), monoclonal

species reactivity

rat, mouse, avian, human, bovine, monkey, pig, sheep

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_006086.2

UniProt accession no.

Q13509

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TUBB3(10381)

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General description

55 kDa
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.

Immunogen

A synthetic peptide corresponding to amino acids 443-450 (ESEAQGPK) of human class III beta-tubulin conjugated to KLH.
Epitope: C-terminus

Application

Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB with more than 55 product citations.
Immunohistochemistry:
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.

Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.

ELISA:
A previous lot of this antibody was used on ELISA.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Biochem/physiol Actions

Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.

Physical form

Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.
Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Replaces: 04-1049

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

T-cell-mediated disruption of the neuronal microtubule network: correlation with early reversible axonal dysfunction in acute experimental autoimmune encephalomyelitis.
Shriver, Leah P and Dittel, Bonnie N
The American Journal of Pathology, 169, 999-1011 (2006)
Laminin-5 activates extracellular matrix production and osteogenic gene focusing in human mesenchymal stem cells.
Robert F Klees, Roman M Salasznyk, Scott Vandenberg, Kristin Bennett, George E Plopper
Matrix Biology null
K A Sharif et al.
Neuroscience, 126(4), 967-978 (2004-06-23)
Laminins are the major glycoproteins present in basement membrane, a type of extracellular matrix. We showed that the LAMB1 gene, which encodes the laminin beta1 subunit, is transcriptionally activated by retinoic acid in embryonic stem cells. However, little information is
BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype.
Silva, Ana, et al.
Journal of Neuroscience Research, 87, 1986-1996 (2009)
Human fibroblast-derived cell lines have characteristics of embryonic stem cells and cells of neuro-ectodermal origin
Rieske, P. et al.
Differentiation, 73(9-10), 474-483 (2005)
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국제 무역 품목 번호

SKUGTIN
MAB163708436037122545

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