Anti-Glutamine Synthetase Antibody, clone GS-6

45 kDa

Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.

Glutamine synthetase purified from sheep brain.

Detect Glutamine Synthetase using this Anti-Glutamine Synthetase Antibody, clone GS-6 validated for use in ELISA, IH, IH(P) & WB with more than 45 product citations.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Western blot: 1:1,000-1:5,000

Immunohistochemistry on rat brain

ELISA

Optimal working dilutions must be determined by end user.

Reacts strongly with a single band on Western blots of sheep brain and rat brain. Glutamine synthetase, an enzyme that catalyzes the amination of glutamic acid to form glutamine, is found in mammals as an octamer of eight identical 45 kDa subunits. Glutamine synthetase activity has been shown to be a useful marker of astrocytes and an important differentiation feature in retina. Glutamine synthetase is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.

Format: Purified

Protein A purified

Purified immunoglobulin. Liquid in buffer containing 50% glycerol, BSA and 0.09% sodium azide.

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.

Control
Rat brain tissue, rat brain cytosolic fraction extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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