Anti-DNA Antibody, single stranded, clone 16-19

Epitope: single stranded DNA

ELISA
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.

Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Cell Cycle, DNA Replication & Repair

Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA.

ANTIGEN BINDING PROFILE:

Relative Binding To:

Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53

a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA

Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).

BINDING ASSAY

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.

ISOELECTRIC POINT = 8.3

0.1 M Tris-Glycine (pH 7.4), 150 nM NaCl with 0.05% sodium azide.

Format: Purified

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

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