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Merck

MAB318

Anti-Tyrosine Hydroxylase Antibody

CHEMICON®, mouse monoclonal, LNC1

동의어(들):

TH, Tyrosine Monooxygenase, Tyrosine 3-monooxygenase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
LNC1, monoclonal
Species reactivity:
zebrafish, mouse, rat, vole, human, frog, lizard, monkey, chicken
Application:
IHC (p), IP, WB
Citations:
782

주요 문서

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제품 이름

Anti-Tyrosine Hydroxylase Antibody, clone LNC1, ascites fluid, clone LNC1, Chemicon®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

LNC1, monoclonal

species reactivity

zebrafish, mouse, rat, vole, human, frog, lizard, monkey, chicken

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_000360.3

UniProt accession no.

P07101

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TH(7054)
rat ... Th(25085)

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General description

59-63 kDa
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Immunogen

Tyrosine Hydroxylase purified from PC12 cells

Application

Detect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.
Immunohistochemistry(paraffin):
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.

Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).

Optimal working dilutions and protocols must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).

Physical form

Ascites mouse monoclonal IgG1κ fluid containing PBS pH 7.1 , contains no preservative.
Unpurified

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
Routinely evaluated by Western Blot on Mouse Brain lysates.

Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Suppression of prolactin-induced signal transducer and activator of transcription 5b signaling and induction of suppressors of cytokine signaling messenger ribonucleic acid in the hypothalamic arcuate nucleus of the rat during late pregnancy and lactation.
Anderson, GM; Beijer, P; Bang, AS; Fenwick, MA; Bunn, SJ; Grattan, DR
Endocrinology null
A FRET-based method to study protein thiol oxidation in histological preparations.
Mastroberardino, PG; Orr, AL; Hu, X; Na, HM; Greenamyre, JT
Free radical biology & medicine null
Expression patterns of the opsin 5-related genes in the developing chicken retina.
Sayuri Tomonari,Kyoichi Migita,Akira Takagi,Sumihare Noji,Hideyo Ohuchi
Developmental Dynamics null
Age and region-specific responses of microglia, but not astrocytes, suggest a role in selective vulnerability of dopamine neurons after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine exposure in monkeys.
Kanaan, NM; Kordower, JH; Collier, TJ
Glia null
Paraskevi Krashia et al.
Nature communications, 10(1), 4725-4725 (2019-10-16)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
모든 관련된 서류 보기

문서

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Antibody Generation | Monoclonal vs Polyclonal Antibodies

Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.

Antibody Basics

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

Antibody Basics

구조, 클래스, 면역글로불린의 정상 범위에 관한 기술 정보 등 항체 작업의 기본 원칙에 대해 알아보십시오.

프로토콜

Tips and Techniques for Troubleshooting Immunohistochemistry (IHC)

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

국제 무역 품목 번호

SKUGTIN
MAB31808436037123320

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