Anti-Growth Associated Protein 43 Antibody, clone 9-1E12

43-48 kDa

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

GAP-43 purified from rat brain

Anti-Growth Associated Protein 43 Antibody, clone 9-1E12 detects level of Growth Associated Protein 43 & has been published & validated for use in IH, IP & WB.

Immunohistochemistry:
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.

Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.

Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuroregenerative Medicine

GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein’s phosphorylation state.

Format: Purified

Protein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Protein A purified

Maintain at 2-8°C in undiluted for up to 6 months after date of receipt.

Control
Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.

Routinely evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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