Anti-macro-H2A1.2 Antibody, clone 14G7

Histone MacroH2A (mH2A), also known as H2AFY, is a vertebrate-specific histone variant associated with condensed, inactive heterochromatin. This histone variant consists of a histone H2A-like domain fused to a large non-histone region. mH2A has been shown to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. The non-histone region of mH2A is directly involved in the repression of transcription, histone acetylation and blocking SWI/SNF and ACF nucleosome remodeling. This variant histone is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci.

~39 kDa observed

GST-tagged recombinant protein corresponding to human macro-H2A1.2.

Anti-macro-H2A1.2 Antibody, clone 14G7 is a Mouse Monoclonal Antibody for detection of macro-H2A1.2 also known as H2A histone family member Y or Histone macroH2A1 & has been validated in WB, ICC & IHC.

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Format: Purified

Protein G

Purified mouse monoclonal IgG2bλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
MCF-7 cell lysate

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected macro-H2A1.2 on 10 µg of MCF-7 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.