M16 Medium

Can be stored at -20°C up to the expiration date on the bottle. Once thawed it should be used within 2 weeks.

Frozen media can be placed in a refrigerator (2°C to 8°C) to thaw overnight. The thawed media should be swirled gently to mix the media, and warmed to 37°C prior to use. Altematively, place frozen medium into a 37°C water bath until thawed completely. It is best to gently swirl the medium during thawing to accelerate the process, and mix the medium. Do not allow the thawed medium to remain in the 37°C water bath for extended periods after it has thawed completely, and warmed to 37°C.

Once thawed the medium has a shelf life of 2 weeks, and must be stored at 2°C to 8°C between uses. Do not re-freeze the medium. Medium stored beyond this 2 week period may not perform optimally.

Note 1:


The "Last Thaw Date" on the label indicates the last day in the shelf life of the frozen media. Media stored frozen beyond this date may not perform optimally. Media are best used prior to the "Last Thaw Date" however, once the "Last Thaw Date" has been reached, the media should be thawed, stored at 2°C to 8°C, and used within 2 weeks.

Note 2:


Although the pH of these media are correct when thawed, the pH of the sodium bicarbonate buffered formulations will rise as the media are exposed to air. This rise in pH will increase with the frequency of opening the bottle, and is particularly rapid when drops of medium placed on a dish for embryo culture are exposed to air. Thus, it is recommended that the drops of medium (placed under light mineral oil) are pre-equilibrated 6 hours to overnight in a humidified incubator at 37°C, in a 5% C02 + 95% Air atmosphere, prior to introducing embryos into the medium.

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.