biological source
bovine intestine
form
suspension
specific activity
~200 units/mg protein (At 25 °C with adenosine as the substrate.)
packaging
pkg of 1 mL (10 mg)
manufacturer/tradename
Roche
technique(s)
activity assay: suitable
color
white
optimum pH
7.0-7.4
solubility
water: miscible
suitability
suitable for chemiluminescence
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
foreign activity
AMP-deaminase <0.01%, AP <0.01% (with pre incubation), Guanase <0.01%, NP <0.01%
storage temp.
2-8°C
Gene Information
bovine ... ADA(280712)
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General description
Adenosine aminohydrolase.
Adenosine deaminase (ADA) is a zinc metalloenzyme.
Adenosine deaminase (ADA) is a zinc metalloenzyme.
Application
Chemiluminescent adenosine assay.
Deamination of adenosine analogs to the corresponding inosine analogs
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.
Adenosine deaminase (ADA) has been used in lipolysis assay along with other reagents.
Biochem/physiol Actions
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Small particles are normal. Before using stir up the precipitate and proceed.
Analysis Note
Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each
Other Notes
For life science research only. Not for use in diagnostic procedures.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
Cultured astrocytes do not release adenosine during hypoxic conditions.
Fujita T, et al.
Journal of Cerebral Blood Flow and Metabolism, 32(1), e1-e7 (2012)
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
Adenosine deaminase from calf intestinal mucosa.
C A ZITTLE
The Journal of biological chemistry, 166(2), 499-503 (1946-12-01)
Sonia Muñoz-López et al.
Frontiers in endocrinology, 13, 1007801-1007801 (2022-11-22)
Despite great efforts, effective treatment against cancer has not yet been found. However, natural compounds such as the polyphenol resveratrol have emerged as promising preventive agent in cancer therapy. The mode of action of resveratrol is still poorly understood, but
Alternative substrates for calf intestinal adenosine deaminase. A pre-steady-state kinetic analysis.
D J Porter et al.
The Journal of biological chemistry, 268(4), 2480-2485 (1993-02-05)
The protein fluorescence of adenosine deaminase (ADA) was perturbed during the deamination of adenosine and four analogues of adenosine. The kinetics for the approach to the steady-state during turnover were monitored by fluorescence changes associated with formation of enzymatic intermediates.
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