biological source
bacterial (Flavobacterium meningosepticum)
recombinant
expressed in E. coli
conjugate
(N-linked)
assay
≥90% (SDS-PAGE)
form
lyophilized
specific activity
>25000 units/mg protein
mol wt
35.5 kDa
purified by
electrophoresis
packaging
pkg of 100 U (11365185001), pkg of 250 U (11365193001)
manufacturer/tradename
Roche
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
technique(s)
activity assay: suitable
color
colorless
optimum pH
7.0-8.0
solubility
water: soluble
suitability
suitable for enzyme test
foreign activity
Endoglycosidase F, none detected, a-Fucosidase, present, b-Galactosidase, present, b-N-Acetylhexosaminidase, present, dA(17h ≤100 units, present
storage temp.
2-8°C
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General description
N-Glycosidase F (PNGase F) is a potent enzyme which hydrolyzes at glycosylamine linkage. It also helps in generating a carbohydrate-free peptide and oligosaccharide with di-N-acetylchitobiose unit.
N-glycosidase F, also known as PNGase F, is an asparagine amidase enzyme derived from Flavobacterium meningosepticum. It is widely used as a valuable tool in protein research to investigate and analyze N-glycosylation.
N-glycosidase F, also known as PNGase F, is an asparagine amidase enzyme derived from Flavobacterium meningosepticum. It is widely used as a valuable tool in protein research to investigate and analyze N-glycosylation.
Application
N-Glycosidase F has been used for deglycosylation of N-glycoproteins.
Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a solution.
Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a solution.
Biochem/physiol Actions
Hydrolyzes all types of N-glycan chains from glycopeptides and glycoproteins unless they carry α1,3-linked core fucose residues present in insect and plant glycoproteins. Free of contaminating proteolytic activities (x = H or sugar[s]) according to current quality control procedures.
Physical form
Clear, colorless solution after reconstitution
Preparation Note
Storage conditions (working solution): 2 to 8 °C
The reconstituted solution is stable at 2 to 8 °C for at least four weeks.
The reconstituted solution is stable at 2 to 8 °C for at least four weeks.
Dissolving the content in 0.1 ml redist water (100 unit package) or 0.25 ml double-dist. water (250 unit package) respectively, results in a concentration of 100 mM sodium phosphate buffer, 25 mM EDTA, pH 7.2.
Note: N-Glycosidase F, recombinant is also available as solution with 50% glycerol.
Note: N-Glycosidase F, recombinant is also available as solution with 50% glycerol.
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycoprotein within 1 minute at 37 °C and pH 7.8.
저장 등급
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
signalword
Warning
hcodes
pcodes
Hazard Classifications
STOT RE 2
A L Tarentino et al.
Biochemistry, 24(17), 4665-4671 (1985-08-13)
Endo-beta-N-acetylglucosaminidase F (Endo F) and peptide:N-glycosidase F (PNGase F) were purified from cultures of Flavobacterium meningosepticum by ammonium sulfate precipitation followed by gel filtration on TSK HW-55(S). This system separated the two enzymes and provided PNGase F in a high
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