form
liquid
Quality Level
shelf life
limited shelf life, expiry date on the label
technique(s)
microbe id | staining: suitable
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
suitability
suitable for microscopy, Bacillus spp., Corynebacterium spp.
General description
Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.
Other Notes
Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water
Nigrosin 100 g/l, Formalin 5 ml/l in water
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Skin Sens. 1
저장 등급
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves
A L Fedorovtsev
Sudebno-meditsinskaia ekspertiza, 35(2), 28-30 (1992-04-01)
The morphology of isolated cells of multi-layer squamous non-horny epithelium (buccal, vaginal, urethral, rectal), stained with 0.05% nigrosine solution, was under study, and the sizes of these cells and their nuclei were measured. Specific markers were detected in the buccal
A Novelli
Pathologica, 88(3), 188-191 (1996-06-01)
Nigrosin, an aqueous blue-black acid dye of the azine series, in conjunction with Biebrich scarlet, orange G and formic acid as mordant, it is found an excellent triple panchromatic rapid stain for histological purposes. The staining so obtained permits a
L Björndahl et al.
Human reproduction (Oxford, England), 18(4), 813-816 (2003-03-28)
The one-step eosin-nigrosin staining technique for assessment of sperm vitality was developed in the 1950s for various mammalian species. Although commonly used on human sperm in semen, a validation for this use has not previously been published. The technique was
Shuai Zhang et al.
Journal of colloid and interface science, 316(1), 168-174 (2007-08-31)
A convenient approach was developed to fabricate monodisperse nigrosine-doped poly(methyl methacrylate-co-divinylbenzene-co-methacrylic acid) nanoparticles with different cross-linkage by soap-free emulsion polymerization at boiling status and swelling process. The dye-doped nanoparticles were used for the fabrication of colloidal crystal films and beads.
D A Hewitt et al.
Animal reproduction science, 51(4), 321-332 (1998-08-01)
The processes of capacitation and acrosomal exocytosis of dog spermatozoa in vitro have yet to be fully investigated. Firstly, we investigated the effectiveness of a technique for staining dog spermatozoa with the fluorescent labels Hoechst 33258 and chlortetracycline. A modified
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