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B0126

Bromophenol Blue

Name: Bromophenol Blue
Brand: SIAL

Powder

동의어(들):

3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₁₉H₁₀Br₄O₅S

CAS 번호:

115-39-9

Molecular Weight:

669.96

NACRES:

NA.47

PubChem Substance ID:

24891504

UNSPSC Code:

12171500

EC Number:

204-086-2

MDL number:

MFCD00005875

Beilstein/REAXYS Number:

61698

기술 서비스

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속성

제품 이름

Bromophenol Blue, titration: suitable

Quality Level

200

agency

suitable for SM 2310, suitable for SM 2320

form

powder

technique(s)

titration: suitable

color

faint beige to very dark beige, faint orange to very dark orange

visual transition interval

3.0-4.6, yellow-green to blue

mp

273 °C

solubility

methanol: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

설명

General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

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저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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